Microrna214 suppresses osteogenic differentiation of. Serum high expression of mir214 and mir5b as novel predictor. An lncrnamrna coexpression network, mirnamrna target prediction network, and. The mir214 inhibits osteoblast activity of activating transcription factor 4 atf4 and regulates the. Micrornas regulate bone development and regeneration.
Mirnas target mrnas via complementary basepairing to multiple sites. Osteoclastderived exosomal mir2143p inhibits osteoblastic bone. Microrna214 suppresses osteogenic differentiation of human. Winter j, jung s, keller s, gregory ri, diederichs s. This article introduces a new function for microrna mirna. For the target of mir214, atf4 protein expression level was decreased after. We prepared cells overexpressing mir 214 and found that mir 214 negatively regulates osteogenic differentiation of hpdlscs. In conclusion, we found that mir 214 atf4 axis is a novel pathway for regulating hpdlsc osteogenic differentiation. Atf6 cleavage merge to regulate xbp1 in signaling the unfolded. The role of micrornas in bone remodeling international journal of. In a new study yingxian li and her colleagues show that the microrna mir 214 targets atf4 in osteoblasts to negatively regulate their activity.
Atf4, a gene encoding one of the main transcription factors required for osteoblast function. We show that mir 214 participates in the inhibition of osteoblast differentiation and osteoblastic bone formation in skeletal disorders. Our previous study also identified that mir2143p could target atf4, an important osteogenic transcriptional factor, to suppress bone formation. Moreover, atf4 can also bind to the promoter of rankl and can ultimately inhibit bone resorption. Taken together, we hypothesized that mir 214 might be a part of the cellular defense system in protecting erthyroid cells against oxidative stressinduced toxicity. A recent study also demonstrated that mir 214 directly targets atf4 to restrain bone formation in osteoclasts by suppressing osteoclast activity. R214 targets atf4 to functionally inhibit osteoblast activity in vitro. For the target of mir 214, atf4 protein expression level was decreased after induction. Bone remodeling is balanced by bone formation and bone resorption as well as by alterations in the. Supplementary figure 1 mirna screening a nd correlation analysis with reduced bone formation in human and mice. Evidences for a new role of mir214 in chondrogenesis. A transcriptomelevel study identifies changing expression. Sigmaaldrich offers abstracts and fulltext articles by xiaogang wang, baosheng guo, qi li, jiang peng, zhijun yang, aiyuan wang, dong li, zhibo hou, ke lv. It is targeted by mir214 that inhibits bone formation in mouse.
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